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Cryo LM of high-pressure-frozen thick samples (C. elegans)
This video describes a workflow to execute cryo fluorescence microscopy on high-pressure-frozen C. elegans worms. This collaboration with Carl Zeiss GmbH was presented (Poster #1085) at Microscopy & Microanalysis 2020.
“2.5D” Neural Network based segmentation of mitochondria
This video describes a method to enforce prediction consistency across orthogonal planes (hence 2.5D) to improve neural network segmentation of mitochondria in a FIB-SEM image volume. This work was presented (poster #831) at Microscopy & MIcroanalysis 2020.
Towards artifact-free FIB-SEM acquisition
This video describes artifact-free FIB-SEM data acquisition strategies, and accompanied a poster (#535) presented at Microscopy & Microanalysis 2020
Editing mitochondrial skeletons in a virtual reality (VR) environment
In this movie, we describe a module in ArivisVR to import, edit and export corrected mitochondrial skeletons. This work (in collaboration with arivis Inc) was presented (Poster #448) at Microscopy & Microanalysis 2020.